Triple Sugar Iron
Note: The following combination of ingredients can substitute for the first two components listed: beef extract, 3.0 g; yeast extract, 3.0 g; and peptone, 20.0 g.
Combine ingredients, and adjust the pH to 7.3. Boil to dissolve the agar, and dispense into tubes. Sterilize by autoclaving at 121°C for 15 min. Cool in a slanted position to give a 2.5-cm butt and a 3.8-cm slant.
TSI agar is also available commercially.
Reference: Gephart, P., R.G. E. Murray, W.A. Wood, and N.R. Krieg. (1994) Methods for General and Molecular Bacteriology, ASM Press, Washington. DC.