Triple Sugar Iron

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Introduction

Triple sugar iron agar (TSIA) is a tubed differential medium used in determining carbohydrate fermentation and H2S production. Gas from carbohydrtate metabolism can also be detected. TSIA contains three carbohydrates: glucose (0.1%), sucrose (1%), and lactose (1%). TSIA is similar to Kligler's iron agar, except that Kligler's iron agar contains only two carbohydrates: glucose (0.1%) and lactose (1%).