Please contact Kathy Coyne for reprints of these publications:

Stewart J.J., Bianco, C. M., Miller, K. R., Coyne, K. J. (2015) The marine microalga, Heterosigma akashiwo, converts industrial waste gases into valuable biomass. Frontiers in Energy Research 3:12. (Link) 

Turner, C. R., Miller, D. J., Coyne, K. J. and Corush, J. (2014) Improved Methods for Capture, Extraction, and Quantitative Assay of Environmental DNA from Asian Bigheaded Carp (Hypophthalmichthys spp.) PLoS ONE 9(12): e114329. (Link) 

Tilney, C.L., Pokrzywinski, K. L., Coyne, K. J., and Warner, M.E. (2014) Effects of a bacterial algicide, IRI-160AA, on dinoflagellates and the microbial community in microcosm experiments. Harmful Algae 30:210-222. (Link)

Keeling, P. J., Burki, F., Wilcox, H. M., Allam, B., Allen, E., Amaral-Zettler, L. A., Armbrust, E. V. and 71 others. (2014) The Marine Microbial Eukaryote Transcriptome Sequencing Project (MMETSP): Illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing. PLOS Biology 12(6):e1001889. (Link)

Main, C. R., Doll, C., Bianco, C., Greenfield, D. I., and Coyne, K. J. (2014) Effects of growth phase, diel cycle and macronutrient stress on the quantification of Heterosigma akashiwo using qPCR and SHA. Harmful Algae 37:92-99. (Link)

Cary, S. C., Coyne, K. J., Rueckert, A., Wood, S. A., Kelly, S., Gemmill, C. E. C., Vieglais, C. and Hicks, B. J. (2014) Development and validation of a quantitative PCR assay for the early detection and monitoring of the invasive diatom Didymosphenia geminata. Harmful Algae 36:63-70. (Link) 

Doll, C., Main, C. R., Bianco, C., Coyne, K. J., Greenfield, D. I. (2014) Comparison of sandwich hybridization assay and quantitative PCR for the quantification of live and preserved cultures of Heterosigma akashiwo (Raphidophyceae). Limnology and Oceanography: Methods 12:232-245. (Link)  

Tilney, C. L., Pokrzywinski, K. L., Coyne, K. J. and Warner, M. E. (2014) Growth, death and photobiology of dinoflagellates (Dinophyceae) under bacterial-algicide control.  Journal of Applied Phycology, 26(5):2117-2127. (Link) 

Coyne, K. J., Countway, P. D., Pilditch, C. A., Lee, C. K., Caron, D. A. and Cary, S. C. (2013) Diversity and distributional patterns of ciliates in Guaymas Basin hydrothermal vent sediments. Journal of Eukaryotic Microbiology 60:433-447. http://dx.doi.org/10.1111/jeu.12051.

Hennige, S. Coyne, K. J., MacIntyre, H., Liefer, J. and Warner, M. E. (2013) The photobiology of Heterosigma akashiwo: Photoacclimation, diurnal periodicity and its ability to rapidly exploit exposure to high light. Journal of Phycology 49 (2):349-360. (Link)

Demir-Hilton, E., Hutchins, D. A., Czymmek, K. J. and Coyne, K. J. (2012)  Description of Viridilobus marinus (gen. et sp. nov.), a new raphidophyte from Delaware's inland bays. Journal of Phycology 48:1220-1231.  (Link)

Pokrzywinski, K. L., Place, A. R., Warner, M. E. and Coyne, K. J.  Investigation of the algicidal exudate produced by Shewanella sp. IRI-160 and its effect on dinoflagellates. Harmful Algae 19:23-29. (Link)

Stewart, J. J. and Coyne, K. J. (2011) Analysis of raphidophyte assimilatory nitrate reductase reveals unique domain architecture incorporating a 2/2 hemoglobin. Plant Molecular Biology 77:565-575. (Link)

Gobler, C. J., Berry, D. L., Dyhrman, S. T., Wilhelm, S. W., Salamov, A., Lobanov, A. V., Zhang, Y., Collier, J. L., Wurch, L. L., Kustka, A. B., Dill, B. D., Shah, M., VerBerkmoes, N. C., Kuo, A., Terry, A., Pangilinan, J., Lindquist, E. A., Lucas, S., Paulsen, I. T., Hatternrath-Lehmann, T. K., Talmage, S. C., Walker, E. A., Koch, F., Burson, A. M., Marcoval, M. A., Tang, Y.-Z., LeCleir, G. R., Coyne, K. J., Berg, G. M., Bertrand, E. M., Saito, M. A., Gladyshev, V. N., Grigoriev, V. N. (2011) Niche strategy of harmful algae revealed through comparative genomics of competing phytoplankton species.  Proceedings of the National Academy of Sciences 108:4352-4357. (Link)

Coyne, K. J. (2010) Nitrate reductase (NR1) sequence and expression in the harmful alga Heterosigma akashiwo (Raphidophyceae).  Journal of Phycology 46: 135-142. (Link)

Portune, K. J., Coyne, K. J., Hutchins, D. A., Handy, S. M. and Cary, S. C.  (2009) Germination of Heterosigma akashiwo and Chattonella subsalsa (Raphidophyceae) cysts in Delaware’s Inland Bays, DE. Aquatic Microbial Ecology 55: 229-239. (Link)

Handy, S. M., Demir, E., Hutchins, D. A., Portune, K. J., Whereat, E. B., Hare, C. E., Rose, J. M., Warner, M., Farestad, M., Cary, S. C., and Coyne, K. J.(2008) Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies. Harmful Algae 7:599-613. (Link)

Demir, E., Coyne, K. J., Doblin, M. E., Handy, S. M. and Hutchins, D. A. (2008) Microzooplankton grazing rates on Heterosigma akashiwo in Delaware Inland Bays: A species-specific approach combining quantitative real-time PCR (QPCR) and dilution methods. Microbial Ecology 55:583-594. (Link)

Gobler, C. J., Davis, T. W., Coyne, K. J., and Boyer, G. L. (2007) The interactive influences of nutrient loading and zooplankton grazing on the growth and toxicity of cyanobacteria blooms in a eutrophic lake.  Harmful Algae 6:119-133. (Link) (Corrigendum)

Coyne, K. J., Hare, C. E., Popels, L. C., Hutchins, D. A., and Cary, S. C. (2006) Distribution of Pfiesteria piscicida cyst populations in sediments of the Delaware Inland Bays, USA. Harmful Algae 5:363-373. (Link)

Handy, S. M., Cary, S. C., Hutchins, D. A., and Coyne, K. J. (2006) Simultaneous enumeration of multiple raphidophyte species by quantitative real-time PCR: capabilities and limitations. Limnol. Oceanog. Methods 4:193-204. (Link)

Zhang, Y., F.-X. Fu, E., Whereat, K.J. Coyne and D. A. Hutchins. (2006) Bottom-up controls on a mixed-species HAB assemblage:  A comparison of sympatric Chattonella subsalsa and Heterosigma akashiwo (Raphidophyceae) isolates from the Delaware Inland Bays, USA.  Harmful Algae 5:310-320. (Link)

Coyne, K. J.,  Handy, S. M., Demir, E., Whereat, E. B.,  Hutchins, D. A.,  Portune, K. J., Doblin, M. E. and Cary, S. C. (2005) Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard. Limnology and Oceanography Methods 3:381-391. (Link)

Handy, S. M., Coyne, K. J., Portune, K. J., Demir, E., Doblin, M. E., Hare, C. E., Cary S. C., and Hutchins, D. A.  (2005) Evaluating vertical migration behavior of harmful raphidophytes in the Delaware Inland Bays utilizing quantitative real-time PCR.  Aquatic Microbial Ecology 40:121-132. (Link) (Erratum)

Coyne, K. J. and S. C. Cary (2005) Molecular approaches to the investigation of viable dinoflagellate cysts in natural sediments from estuarine environments. Journal of Eukaryotic Microbiology 52:90-94. (Link)

Burkholder, J. M., A. S. Gordon, P. D. R. Moeller, J. M. Law, K. J. Coyne, A. J. Lewitus, J. S. Ramsdell, H. G. Marshall, N. J. Deamer,  S. C. Cary, J. W. Kempton, S. L. Morton, and P. A. Rublee (2005) Demonstration of toxicity to fish and to mammalian cells by Pfiesteria species: Comparison of assay methods and strains. PNAS 102: 3471–3476. (Link)

Hare, C.E., Demir, E., Coyne, K.J., Cary, S.C. and Hutchins, D.A. (2005) A bacterium from the Delaware Inland Bays that inhibited the growth of Pfiesteria piscicida and other dinoflagellates. Harmful Algae 4:221-234. (Link)

Doblin, M. E., L. A. Drake, K. J. Coyne, P. A. Rublee and F. C. Dobbs. (2004) Pfiesteria species identified in ships' ballast water and residuals: a possible vector for introductions to coastal areas, pp. 317-319. In K. A. Steidinger, J. H. Landsberg, C. R. Tomas and G. A. Vargo (eds) Harmful Algae 2002. Florida Fish and Wildlife Conservation Commission, Florida Institute of Oceanography, and Intergovernmental Oceanographic Commission of UNESCO.

Doblin, M.E., Popels, L.C., Coyne, K.J., Hutchins, D.A., Cary, S.C. and Dobbs, F.C. (2004) Long-distance and local-scale transport of Aureococcus species by ships and boats in fresh and marine waters. Appl Environ Microbiol 70:6495-6500. (Link)

Coyne, K. J., J.A. Burkholder, R. A. Feldman, D. A. Hutchins and S. C. Cary. (2004) Modified Serial Analysis of Gene Expression (SAGE) method for construction of gene expression profiles of microbial eukaryotic species. Appl Environ Microbiol 70:5298–5304. (Link)

Popels, L. C., S. C. Cary, D. A. Hutchins, R. Forbes, F. Pustizzi, C. J. Gobler, and K. J. Coyne (2003) The use of quantitative polymerase chain reaction for the detection and enumeration of the harmful alga anophagefferensAureococcus in environmental samples along the United States East Coast. Limnology and Oceanography Methods 1:92-102. (Link)

Coyne, K. J., D. A. Hutchins, C. E. Hare and S. C. Cary  (2001)  Assessing temporal and spatial variability in Pfiesteria piscicida distributions using molecular probing techniques.  Aquatic Microbiol Ecol  24:275-285. (Link)