CHEM-342 Introduction to Biochemistry        Group Members ________________________
Final Examination - Group Part
Tuesday, 21 May 2002                                                          ________________________
8:45 - 10:00 PM
H. B. White - Instructor                                                         ________________________


Important - Please read this before you turn the page.

Iron Deficiency Anemia???

The atomic mass of iron is the weighted average of the masses of its isotopes of which the four common non-radioactive ones are listed below from the 77th Edition of the CRC Handbook of Chemistry and Physics.

% Natural Abundance

Mass spectrometry can measure the relative abundance of each isotope in various samples with great accuracy and precision. Thus, differences in isotope abundance among samples of as little as 0.01% can be detected. The figure below was published about two months ago in Science [1]. In a single graph it depicts the relative abundance of the three most common iron isotopes in blood samples taken from 15 men and 29 women. For example, a delta 57Fe (‰) value of -3 on the y-axis means that there is a 3 part per thousand (0.3%) deficit of 57Fe in an iron sample relative to 54Fe. A reference standard with the natural abundances listed above would be a point at the origin in the upper right hand corner.
56Fe (‰)  ={ [(56Fe/54Fe)sample/(56Fe/54Fe)std] – 1} x 1000

57Fe  (‰) ={ [(57Fe/54Fe)sample/(57Fe/54Fe)std] – 1} x 1000

1. (15 points) After generating a significant list of group learning issues on a separate sheet, organize the list into five major categories and present them on the next page in a coherent way that displays logic and depth. 

2. (10 points) Generate a testable hypothesis built around one of your learning issues and, based on your knowledge and intuition, make a prediction of the outcome.

[1] Thomas Walczyk and Friedhelm von Blanckenburg, “Natural Iron Isotope Variations in Human Blood,” Science 295, 2065-2066 (2002)

Learning Issue Category 1 ___________________________________

Learning Issue Category 2 ___________________________________

Learning Issue Category 3 ___________________________________

Learning Issue Category 4 ___________________________________

Learning Issue Category 5 ___________________________________

Testable hypothesis generated by one category of learning issues.

Prediction of the results of a test of your hypothesis.

Bonus Question
(5 points) Work on this problem only if your group has finished the main problem. The average life span of a red blood cell is about 4 months. Estimate to within one order of magnitude the number of hemoglobin molecules your body makes every second.

Return to Hal White's Home Page, Course Home Page, or Departmental Home Page.
Posted 23 May 2002 by Hal White
Copyright 2002, Harold B. White, Department of Chemistry and Biochemistry, University of Delaware