BISC411
EXPERIMENTAL MOLECULAR BIOLOGY OF THE CELL
Welcome to our course homepage. From this
docking
point you will be able to access the laboratory schedule,
protocols,
etc. for our course. There will not be a laboratory manual. Please take
some time to
familiarize yourself with the background
information
about the course, the course philosophy, etc. We will be
having extensive discussions related to the
actual
lab protocols as part of our course so you should not be
concerned if your initial reading of the
protocols
seems unclear. I hope you will enjoy this semester and find the course
a worthwhile experience.
Course Objectives and Background
General Schedule and Grading Criteria
Guidelines for the Laboratory Reports
Using the PDB to Examine T4 Lysozyme Structure
Background on Restriction Mapping and Pencil Exercise/Questions
Mini-Prep
Protocol and Measurement of DNA yield
Competent Cells and Bacterial
Transformation Protocols
Information
About Alkaline Lysis For Plasmid DNA Purification
Restriction Enzyme Digestion and Agarose Gel Protocols
Determination
of Lysozyme Activity Standard
Ion-Exchange Purification Protocol
Background
Information About Polyacrylamide Gel Electrophoresis
Polyacrylamide Gel Electrophoresis Analysis Protocol
Measuring
Total Protein Concentration (Biuret Reaction) and Units of Lysozyme
Activity
Protocols