Techniques in Biological
Electron Microscopy 

Techniques in Biological Electron Microscopy (B 617) is a graduate -level laboratory course
offered each spring semester to 10 students by permission of intructor only. Students
obtain hands-on experience in preparation of tissue samples for Transmission and Scanning
Electron Microscopy, recording of images by microscopy and presentation of results in a
publication format.
For More Information Contact-   Dr. Roger C. Wagner (instructor)
This page was prepared by Anuj Parikh, AS98, JMC2002

Lecture Topics 
(click on topic to review notes)
Preparation of Tissues for Transmission Electron Microscopy Ultrastructural Cytochemistry Freeze-Fracture and Etching
Ultramicrotomy and Staining of Thin Sections Tracers and Labels for Transmission EM Morphometric Techniques
Transmission Electron Microscopy Negative Staining and Shadow Casting Three-Dimensional Reconstruction
Preparation of Tissues for Scanning Electron Microscopy Corrosion Casting High Resolution Radioautography
Scanning Electron Microscopy Stereomicroscopy Digital Image Processing and Analysis
Photography and Darkroom Techniques Cryofixation Analytical Electron Microscopy