DRI Cytomechanics Core

Researchers at the Delaware Rehabilitation Institute will pursue a multidisciplinary, collaborative approach to address the cellular level of osteoarthritis (OA) along with prevention and treatment. The continuation of this pursuit is afforded by COBRE funding through the National Institutes of Health.

The overall goal of the proposed COBRE is to expand and develop the research capabilities and infrastructure for University of Delaware faculty to enable a multi-scale approach, addressing the problem from a cellular level (Cythomechanics Core) to a larger system’s scale focusing on muscle and joint function (Patient-Specific Modeling Core).

Our Services

  • Confocal imaging
  • Bio-imaging
  • Live-cell imaging
  • Mechanical loading
  • Mechanical testing
  • Bone and Cartilage processing and histology

Our Equipment

Epifluorescence Microscopes: Upright Zeiss w/ Color Axio CamMRc5 camera; Inverted Zeiss w/ Monochrome AxioCamNRc camera and motorized stage; Upright Zeiss Image A2 w/ Color Axio CamMRc5 camera.

Confocal Microscope: Zeiss LSM510 Inverted Observe.Z1 with optional lens inverter, environmental chamber, and heated stage.

Fluorescent Microscope and Calcium Imaging: Leica DMI6000B Inverted w/ high speed EMCCD camera, motorized stage and environmental chamber

Stereomicroscope: Olympus SZx7

Live imaging Apparatus: Isofluorane anesthesia machine; Environmental Chamber; Incubation System (PECON System 2000)

Mechanical Testing Devices: Instron 5848 MicroTester; TA Instruments Dynamic Mechanical Analyzer; Bose ElectroForce TestBench; Custom Piezo-positioning/loading system; Custom Cantilever loading device; Custom Microindenter;

Physiological Manipulation Devices: Syringe Pump (Harvard Apparatus PhD Ultra); MultiChannel Dispenser (ISMATE IPC High Precision); Catheter Blood Pressure measuring system (Millar); Manipulators (Narishig);

Sample Prep and Histology: Diamond Saws (Buehler Isomet 1000 and Isomet low speed precision); 3D table-top milling machine (Roland);

There are multiple ways to take advantage of the array of Cytomechanics Core services. Contact Liyun Wang, Director, to find out more.

Category Item description Vendor Specs Room
RT-PCR Real-time PCR system Applied Biosystems 96-well, 0.2mL format, interactive touchscreen; OptiFlex® technology (featuring 4 coupled channels and white LED), 3 independent Veriflex® temperature zones, cloud-based software SPL203
MicroCT Scanning Scanner uCT35 Scanco Three X-ray energy level; voxel resolution up to 3 micron; field of view up to 70 mm; various sized of holders; controller for imaging/reconstruction/evaluation software; remote access; 10TB storage; tape driver for backup. SPL203
Post-processing suite custom Three networked high-speed PC (Intel Core i7) installed with Simpleware ScanIP (converting microCT to FEM meshes), Hypermesh (for meshing and elastic FEA package), and freeware FEBio (for biphasic and nonlinear FEA) SPL203

Voucher Program

The CytoCore Voucher Program is designed to provide use of the DRI Cytomechanics Core for faculty who need bridge or pilot funding. The program provides fee to cover the cost of using core facilities. Our goal is to help faculty between grants or just starting new projects, enabling them to collect sufficient data for competitive proposals and not loose ground on their research when grants expire.

There are no due dates for proposals. Vouchers are valid for a period of not more than one year and may be renewed. Voucher applications should be submitted to Karen Hough at khough@udel.edu and will be reviewed by Tom Buchanan, DRI Director, and/or Randy Duncan and Liyun Wang, Co-directors for the CytoCore.

All questions should be submitted to Karen Hough or Tom Buchanan (buchanan@udel.edu).

Voucher Program Application (DOC)