ROBERTA F. COLMAN
SELECTED PUBLICATIONS
(Since 1985 - Beginning with No. 98)
- Mas, M. T. and Colman, R. F.: Spectroscopic Studies of the Interactions
of Coenzymes and Coenzyme Fragments with Pig Heart, Oxidized TPN Specific
Isocitrate Dehydrogenase. Biochemistry 24: 1634 (1985).
- Bailey, J. M. and Colman, R. F.: Affinity Labeling of NADP+-Specific Isocitrate
Dehydrogenase by a New Fluorescent Nucleotide Analogue, 2-[(4-Bromo-2,3- dioxobutyl)thio]-1,N6-ethenoadenosine
2',5'-Bisphosphate. Biochemistry 24: 5367 (1985).
- Ehrlich, R. S. and Colman, R. F.: 1H Nuclear Magnetic Resonance
Studies of the Conformation and Environment of Nucleotides Bound to Pig Heart
NADP+-Dependent Isocitrate Dehydrogenase. Biochemistry 24: 5378 (1985).
- Kapetanovic, E., Bailey, J. M. and Colman, R. F.: 2-[(4-Bromo-2,3-dioxobutyl)thio]adenosine
5'-Monophosphate, a New Nucleotide Analogue That Acts as an Affinity Label
of Pyruvate Kinase. Biochemistry 24: 7586 (1985).
- DeCamp, D. L. and Colman, R. F.: Identification of Tyrosine and Lysine
Peptides Labeled by 5'-p-Fluorosulfonylbenzoyl Adenosine in the Active Site
of Pyruvate Kinase. J. Biol. Chem. 261: 4449 (1986).
- Figures, W. R., Scearce, L. M., Wachtfogel, Y., Chem, J., Colman, R. F.
and Colman, R. W.: Evidence for an ADP Requirement for Epinephrine Induced
Platelet Activation and an Influence of Epinephrine on ADP Binding. J. Biol.
Chem. 261: 5981 (1986).
- Batra, S. P. and Colman, R. F.: Isolation and Identification of Cysteinyl
Peptide Labeled by 6-[(4-Bromo-2,3-Dioxobutyl)thio]-6-Deaminoadenosine 5'-Diphosphate
in the DPNH Inhibitory Site of Glutamate Dehydrogenase. Biochemistry 25: 3508
(1986).
- Huang, Y.-C., Bailey, J. M. and Colman, R. F.: Inactivation of NAD-Dependent
Isocitrate Dehydrogenase by Affinity Labeling of the Allosteric ADP Site by
2-(4-Bromo-2,3-dioxobutylthio)-Adenosine 5'-Diphosphate. J. Biol. Chem. 261:
14100 (1986).
- Lark, R. H. and Colman, R. F.: Reaction of the 2',3'-Dialdehyde Derivative
of NADPH at a Nucleotide Site of Bovine Liver Glutamate Dehydrogenase. J.
Biol. Chem. 261: 10659 (1986).
- Batra, S. P. and Colman, R. F.: Affinity Labeling of an Allosteric ADP
Site of Glutamate Dehydrogenase by 2-(4-Bromo-2,3-dioxobutylthio)adenosine
5'-Monophosphate. J. Biol. Chem. 261: 15565 (1986).
- Ehrlich, R. S. and Colman, R. F.: Ionization of Isocitrate Bound to Pig
Heart NADP+-Dependent Isocitrate Dehydrogenase: A 13C NMR Study
of Substrate Binding. Biochemistry 26: 3461 (1987).
- Bailey, J. M. and Colman, R. F. Distances Among Coenzyme and Metal Sites
of NADP+-Dependent Isocitrate Dehydrogenase Using Resonance Energy Transfer.
Biochemistry 26: 4893 (1987).
- Ehrlich, R. S. and Colman, R. F. Characterization of an Active Site Peptide
Modified by the Substrate Analogue 3-Bromo-2-ketoglutarate on a Single Chain
of Dimeric NADP+-Dependent Isocitrate Dehydrogenase. J. Biol. Chem. 262: 12614
(1987).
- Bailey, J. M. and Colman, R. F.: Isolation of the Glutamyl Peptide Labeled
by the Nucleotide Analogue 2-(4-Bromo-2,3-dioxobutylthio)-1,N6-ethenoadenosine
2',5'-Bisphosphate in the Active Site of NADP-specific Isocitrate Dehydrogenase.
J. Biol. Chem. 262: 12620 (1987).
- Bailey, J. M. and Colman, R. F.: 2-(4-Bromo-2,3-dioxobutylthio) and 2-(3-Bromo-
2-oxopropylthio)adenosine 2',5'-Bisphosphate: New Nucleotide Analogues That
Act as Affinity Labels of NADP+-Specific Isocitrate Dehydrogenase. Biochemistry
26: 6858 (1987).
- Saha, A. and Colman, R. F.: Modification of NAD-Dependent Isocitrate Dehydrogenase
by the 2',3'-Dialdehyde Derivatives of NAD, NADH, NADP and NADPH. Arch. Biochem.
Biophys. 262: 665 (1988).
- Colman, R. W., Figures, W. R., Wu, Q-X., Chung, X.-Y., Morinelli, T. A.,
Tuszynski, Colman, R. F. and Niewiarowski, S.: Distinction Between Glycoprotein
IIIa and the 100 kDa Membrane Protein (Aggregin) Mediating ADP-Induced Platelet
Activation. Arch. Biochem. Biophys. 262: 298 (1988).
- DeCamp, D. L., Lim, S. and Colman, R. F.: Reaction of Pyruvate Kinase with
the New Nucleotide Affinity Labels 8-[(4-Bromo-2,3-Dioxobutyl)thio]adenosine
5'-Diphosphate and Triphosphate. Biochemistry 27: 7651 (1988).
- Colman, R. W., Puri, R. N., Zhou, F. and Colman, R. F. Aggregin: The Platelet
Receptor Mediating Activation by ADP in Platelet Membrane Receptors, ed.,
G. A. Jamison, Alan R. Liss, Inc., New York. 1988. P. 263-277.
- Colman, R. F.: Affinity Labels and Spectroscopic Probes of Porcine Heart
NADP-Dependent Isocitrate Dehydrogenase. Biochem. Soc. Trans. 17: 307 (1989).
- Colman, R. F. Affinity Labeling, in Protein Structure and Function: A Practical
Approach, ed., T. E. Creighton, IRL Press. 1989. Chapt. 4, P. 77.
- Batra, S. P., Lark, R. H, and Colman, R. F.: Identification of Histidyl
Peptide Labeled by 2-(4-Bromo-2,3-dioxobutylthio)adenosine 5'-Monophosphate
in an ADP Regulatory Site of Glutamate Dehydrogenase. Arch. Biochem. Biophys.
270: 277 (1989).
- Ehrlich, R. S. and Colman, R. F.: Multinuclear NMR Studies of the Divalent
Metal Binding Site of NADP-Dependent Isocitrate Dehydrogenase from Pig Heart.
Biochemistry 28: 2058 (1989).
- De Camp, D. L. and Colman, R. F.: 2-(4-Bromo-2,3-dioxobutyl)thio]-1,N6-ethenoadenosine
5'-Diphosphate: A New Fluorescent Affinity Label of a Tyrosyl Residue in the
Active Site of Rabbit Muscle Pyruvate Kinase. J. Biol. Chem. 264: 8430 (1989).
- Bansal, A., Dayton, M. A., Zalkin, H. and Colman, R. F.: Affinity Labeling
of a Glutamyl Peptide in the Coenzyme Binding Site of NADP+-Specific Glutamate
Dehydrogenase of Salmonella typhimurium by 2-[(4-Bromo-2,3-dioxobutyl)thio]-1,N6-Ethenoadenosine-
2',5'-Bisphosphate. J. Biol. Chem. 264: 9827 (1989).
- Puri, R. N., Zhou, F., Bradford, H., Hu, C-J., Colman, R. F. and Colman,
R. W.: Thrombin-Induced Platelet Aggregation Involves an Indirect Proteolytic
Cleavage of Aggregin by Calpain. Arch. Biochem. Biophys. 271: 346 (1989).
- Huang, Y.-C. and Colman. R. F.: Aspartyl Peptide Labeling by 2-(4-Bromo-2,3-dioxobutylthio)adenosine
5'-Diphosphate in the Allosteric ADP Site of Pig Heart NAD+-Dependent Isocitrate
Dehydrogenase. J. Biol. Chem. 264: 12208 (1989).
- Saha, A., Huang, Y-C. and Colman, R. F.: Cysteinyl Peptide Labled by 3-Bromo-2-ketoglutarate
in the Active Site of Pig Heart NAD-Dependent Isocitrate Dehydrogenase. Biochemistry
28: 8425 (1989).
- Puri, P. N., Zhou, F. and Colman, R. F.: Cleavage of 100 kDa Membrane Protein
(Aggregin) During Thrombin-Induced Platelet Aggregation is Mediated by the
High Affinity Thrombin Receptors. Biochem. Biophys. Research Commun. 162:
1017 (1989).
- Dombrowski, K. E. and Colman, R. F.: 5'-p-Fluorosulfonylbenzoyl-8-Azidoadenosine:
A New Bifunctional Affinity Label for Nucleotide Binding Sites in Proteins.
Archives Biochem. Biophys. 275: 302 (1989).
- Colman, R. W., Puri, R. N., Zhou, F. and Colman, R. F.: Mechanism of Plasmin-
Induced Platelet Aggregation in "Platelets and Vascular Occlusion." Patrono,
C. and Fitzgerald, ed. Sorono Symposium. Raven Press 54: 279 (1989).
- Colman, R. F.: Glutamate Dehydrogenase (Bovine Liver) in A Study of Enzymes.
Vol. II. Mechanism of Enzyme Action, ed. by S. A. Kuby. C.R.C. Press, 1991,
Chapt. 7, P. 173-192.
- Colman, R. F.: Site-Specific Modification of Enzyme Sites. Chapt. 7. The
Enzymes, 3rd Edition, Volume 19, 1990, P. 283-321.
- Grant, P. G., DeCamp, D. L., Bailey, J. M., Colman, R. W. and Colman, R.
F.: Three New Potential cAMP Affinity Labels; Inactivation of Human Platelet
Low Km cAMP Phosphodiesterase by 8-[(4-Bromo-2,3-dioxobutyl)-Thio]Adenosine
3',5'-Cyclic Monophosphate. Biochemistry 29: 887 (1990).
- Lark, R. H. and Colman, R. F.: Distance Between the Substrate and Regulatory
Reduced Coenzyme Binding Sites of Bovine Liver Glutamate Dehydrogenase by
Resonance Energy Transfer. Eur. J. Biochem. 188: 377 (1990).
- Vollmer, S. H. and Colman, R. F.: Cysteinyl Peptides of Rabbit Muscle Pyruvate
Kinase Labeled by the Affinity Label 8-[(4-Bromo-2,3-dioxobutyl)thio]adenosine
5'-Triphosphate. Biochemistry 29: 2495 (1990).
- Colman, R. F., Bailey, J. M., DeCamp, D. L., Huang, Y-C. and Vollmer, S.
H.: Affinity Labeling of Adenine Nucleotide Sites in Enzymes. Proceedings
of the N. Y. Acad. of Sciences, 603: 417-426 (1990).
- Colman, R. F.: Affinity Labeling of Nucleotide Binding Sites of Enzymes
and Platelets in Fibrinogen, Thrombosis, Coagulation and Fibrinolysis, ed.,
C.-Y. Liu and S. Chien. Plenum Publishing Corp. (1990), P. 257-263.
- Ehrlich, R. S. and Colman, R. F.: Conformations of the Coenzymes and the
Allosteric Activator, ADP, Bound to NAD+-Dependent Isocitrate Dehydrogenase
from Pig Heart. Biochemistry 29: 5179 (1990).
- Ozturk, D. H., Safer, D. and Colman, R. F.: Affinity Labeling of Bovine
Liver Glutamate Dehydrogenase by 8-(Bromo-2,3-dioxobutylthio)adenosine 5'-Diphosphate
and 5'-Triphosphate. Biochemistry 29: 7112 (1990).
- Huang, Y.-C. and Colman, R. F.: Subunit Location and Sequences of the Cysteinyl
Peptides of Pig Heart NAD-Dependent Isocitrate Dehydrogenase. Biochemistry
29: 8266 (1990).
- Puri, R. N., Zhou, F., Colman, R. F. and Colman, R. W.: Plasmin-induced
Platelet Aggregation is Accompanied by Cleavage of Aggregin and Indirectly
Mediated by Calpain. Am. Journal of Physiology 259: C862-C868 (1990).
- Puri, R. N., Zhou, F., Hu, C.-J., Colman, R. F. and Colman, R. W.: High
Molecular Weight Kininogen Inhibits Thrombin-Induced Platelet Aggregation
and Cleavage of Aggregin by Inhibiting Binding of Thrombin to Platelets. Blood,
77: 500 (1991).
- Haeffner-Gormley, L., Chen, Z., Zalkin, H. and Colman, R. F.: Evaluation
of Cysteine-283 and Glutamic Acid-284 in the Coenzyme Binding Site of Salmonella
typhimurium Glutamate Dehydrogenase by Site Directed Mutagenesis and Reaction
with the Nucleotide Analogue 2-[(4-Bromo-2,3-dioxobutyl)thio]-1,N6-ethenoadenosine
2',5'-Diphosphate. J. Biol. Chem. 266: 5388 (1991).
- Smyth, G. E. and Colman, R. F.: Cysteinyl Peptides of Pig Heart NADP-Dependent
Isocitrate Dehydrogenase which are Modified Upon Inactivation by N-Ethylmaleimide.
J. Biol. Chem. 266: 14918 (1991).
- Ozturk, D. H. and Colman, R. F.: Identification of Cysteine-319 as the
Target Amino Acid of 8-[(4-Bromo-2,3-dioxobutyl)thio]adenosine 5'-Triphosphate
in Bovine Liver Glutamate Dehydrogenase. Biochemistry 30: 7126 (1991).
- Katusz, R. M. and Colman, R. F.: S-(4-Bromo-2,3-dioxobutyl)glutathione:
A New Affinity Label for the 4-4 Isoenzyme of Rat Liver Glutathione S-Transferase.
Biochemistry 30: 11230 (1991).
- Grant, P. G., DeCamp, D. L., Bailey, J. M., Colman, R. F. and Colman, R.
W.: Low Km cAMP Phosphodiesterase from Human Platelets: Stimulation of Activity
by Phosphorylation of the Enzyme and Affinity Labeling of the Active Site.
Advances in Second Messenger and Phosphoprotein Research 25: 73-85 (1992).
- Haeffner-Gormley, L., Chen, Z., Zalkin, H. and Colman, R. F.: Reaction
of the Nucleotide Analogue 2-[(4-Bromo-2,3-dioxobutyl)thio]adenosine 2',5'-Bisphosphate
at the Coenzyme Site of Wild Type and Mutant NADP+-Specific Glutamate Dehydrogenases
from Salmonella typhimurium. Archives Biochem. Biophys. 292: 179 (1992).
- Smyth, G. E. and Colman, R. F.: Inactivation of Pig Heart NADP-Specific
Isocitrate Dehdyrogenase by Two Affinity Reagents is Due to Reaction with
a Cysteine Not Essential for Function. Archives Biochem. Biophys. 293: 356
(1992).
- Mills, D.C.B., Puri, R., Hu, C.-J., Minniti, C., Grana, G., Freedman, M.
D., Colman, R. F. and Colman, R. W.: Clopidogrel Inhibits the Binding of ADP
Analogues to the Receptor Mediating Inhibition of Platelet Adenylate Cyclase.
Arteriosclerosis and Thrombosis 12: 430-436 (1992).
- Vollmer, S. H. and Colman, R. F.: Cysteinyl Peptides Labeled by Dibromobutanedione
in Reaction with Rabbit Muscle Pyruvate Kinase. Protein Science 1: 678-687
(1992).
- Dombrowski, K. E., Huang, Y.-C. and Colman, R. F.: Identification of Amino
Acids Modified by the Bifunctional Affinity Label 5'-p-(Fluorosulfonylbenzoyl)-8-azidoadenosine
in the Reduced Coenzyme Site of Bovine Liver Glutamate Dehydrogenase. Biochemistry
31: 3785-3793 (1992).
- Haselbeck, R. J., Colman, R. F. and McAlister-Henn, L.: Isolation and Sequence
of a cDNA Encoding Porcine Mitochondrial NADP-Specific Isocitrate Dehydrogenase.
Biochemistry 31: 6219-6223 (1992).
- Haeffner-Gormley, L., Chen, Z., Zalkin, H. and Colman, R. F.: Importance
of Lysine-286 at the NADP Site of Glutamate Dehydrogenase from Salmonella
typhimurium. Biochemistry 31: 7807-7814 (1992).
- Katusz, R. M., Bono, B. and Colman, R. F.: Identification of Tyr115 Labeled
by S-(4-Bromo-2,3-dioxobutyl)glutathione in the Hydrophobic Substrate Binding
Site of Glutathione S-Transferase, Isoenzyme 3-3. Arch. Biochem. Biophys.
298: 667-677 (1992).
- Katusz, R. M., Bono, B. and Colman, R. F.: Affinity Labeling of Cys111
of Glutathione S-Transferase, Isoenzyme 1-1, by S-(4-Bromo-2,3-dioxobutyl)glutathione.
Biochemistry 31: 8984-8990 (1992).
- Ozturk, D. H., Park, I. and Colman, R. F.: Guanosine-5'-O-[S-(3-Bromooxopropyl)]
Thiophosphate: A New Reactive Purine Nucleotide Analog Labeling Met-169 and
Tyr-262 in Bovine Liver Glutamate Dehydrogenase. Biochemistry 31: 10544-10555
(1992).
- Figures, W. R., Scearce, L. M., Colman, R. F. and Colman, R. W.: Interaction
of Nucleotide Affinity Analog 5'-p-Fluorosulfonylbenzoyl Adenosine with Platelet
ADP Receptor: Aggregin. Methods in Enzymology 215b: 143-155 (1992).
- Ehrlich, R. S. and Colman, R. F.: Selectivity in the Binding of NAD(P)+
Analogues to NAD and NADP-Dependent Pig Heart Isocitrate Dehydrogenases. A
Nuclear Magnetic Resonance Study. Biochemistry 31: 12524 (1992).
- Soundar, S. and Colman, R. F.: Identification of Metal-Isocitrate Binding
Site of Pig Heart NADP-Specific Dehydrogenase by Affinity Cleavage of the
Enzyme by Fe2+-Isocitrate. J. Biol. Chem. 268: 5264 (1993).
- Inshik Park, I., Ozturk, D. H., Soundar, S. and Colman, R. F.: Nicotinamide
Ribose 5'-O-[S-(3-Bromo-2-oxopropyl)thiophosphate: A New Affinity Label for
NMN Sites in Enzymes. Arch. Biochem. Biophys. 303: 483 (1993).
- Colman, R. F.: Targeting Enzyme Sites in Pyruvate Kinase, Glutamate Dehydrogenase
and Glutathione S-Transferase in Protein Structure-Function Relationship ed.
by Zaidi, Z. H., Abbasi, A. and Smith, D. L. Twel Publishers, Karachi. 1993.
P. 39-59.
- Zhou, G., Charbonneau, H., Colman, R. F. and Zalkin, H.: Identification
of Sites for Feedback Regulation of Glutamine 5-Phosphoribosylpyrophosphate
Amidotransferase by Nucleotides and Relationship to Residues Important for
Catalysis. J. Biol. Chem. 268: 10471 (1993).
- Barycki, J. J. and Colman, R. F.: Affinity Labeling of Glutathione S-Transferase,
Isozyme 4-4, by 4-(Fluorosulfonyl)benzoic Acid Reveals Tyr115 to be an Important
Determinant of Xenobiotic Substrate Specificity. Biochemistry 32: 13002-13011
(1993).
- Kumar, A. and Colman, R. F.: 8-(4-Bromo-2,3-dioxobutylthio)NAD: A New Affinity
Label for NAD-Specific Isocitrate Dehydrogenase. Arch. Biochem. Biophys. 308:
357-366 (1994).
- Colman, R. F.: Targeting Enzyme Sites in Pyruvate Kinase, Glutamate Dehydrogenase
and Glutathione S-Transferase. Pure & Appl. Chem. 66: 15 (1994).
- Vollmer, S. H., Walner, M. B., Tarbell, K. V. and Colman, R. F.: Guanosine
5'-0-[S-(4-Bromo-2,3-dioxobutyl)]thiophosphate and Adenosine 5'-0-[S-(4-Bromo-2,3-
dioxobutyl)]thiophosphate: New Nucleotide Affinity Labels which React with
Rabbit Muscle Pyruvate Kinase. J. Biol. Chem. 269: 8082-8090 (1994).
- Wrzeszczynski, K. and Colman, R. F.: Irreversible Activation of Glutamate
Dehydrogenase by Covalent Modification of an Allosteric ADP Site by Adenosine
5'-0-[S-(4-Bromo-2,3-dioxobutyl)]thiophosphate. Biochemistry 33: 11544-53
(1994).
- Colman, R. F.: Affinity Labeling and Related Approaches to Targeting Specific
Sites, Chapt. 7 in Sub-cellular Biochemistry: Structure, Function and Protein
Engineering ed. Biswas, B. B. and Roy, S., Plenum Press, New York, 1995. P.
177-205.
- Huang, Y.-C., Haselbeck, R. J., McAlister-Henn, L. and Colman, R. F.: N-Ethylmaleimide
Profiling of Yeast NADP-Dependent Isocitrate Dehydrogenase. Arch. Biochem.
Biophys. 316: 485-492 (1995).
- Ehrlich, R. S. and Colman, R. F.: 113Cd and 25Mg
NMR Study of the Divalent Metal Binding Sites of Isocitrate Dehydrogenases
from Pig Heart. Biochim. Biophys. Acta 1246: 135-141 (1995).
- Hu, L. and Colman, R. F.: Monobromobimane as an Affinity Label of the Xenobiotic
Binding Site of Rat Glutathione S-Transferase, 3-3. J. Biol. Chem. 270: 21875-83
(1995).
- Puri, R., Kumar, A., Chen, H. and Colman, R. F.: Inhibition of ADP-Induced
Platelet Responses by Covalent Modification of Aggregin, an ADP Receptor,
by 8-(4-Bromo- 2,3-dioxobutylthio)-ADP. J. Biol. Chem. 270: 24482-88 (1995).
- Gite, S. U. and Colman, R. F.: Affinity Labeling of the Active Site of
Rabbit Muscle Adenylosuccinate Lyase by 2-[(4-Bromo-2,3-dioxobutyl)thio] Adenosine
5'-Monophosphate. Biochemistry 35: 2658-2667 (1996).
- Puri, R. N., Colman, R. F. and Colman, R. W.: Platelet Activation by 2-(4-Bromo-2,3-
dioxobutylthio)adenosine 5'-Diphosphate is Mediated by its Binding to the
ADP Receptor, Aggregin. Eur. J. Biochem. 236: 862-870 (1996).
- Wang, J., Barycki, J. J. and Colman, R. F.: Tyrosine-8 Contributes to Catalysis
But is not Essential for Activity of Rat Liver Glutathione S-Transferase,
Isoenzyme 1-1. Protein Science 5: 1032-1042 (1996).
- Colman, R. F.: Affinity Labeling, in Protein Function: A Practical Approach,
ed. by T. E. Creighton, Oxford University Press, Oxford, 1997, Chapt. 6, 155-183.
- Moe, O. A., Baker-Malcolm, J. F., Wang, W., Kang, C., Fromm, H. J. and
Colman, R. F.: Involvement of Arg-143 in Nucleotide Substrate Binding at the
Active Site of Adenylosuccinate Synthetase from E. coli. Biochemistry 35:
9024-9033 (1996).
- Endrizzi, J., Zhang, G., Chen Xiong, W., Hurley, J. H., Remington, S. J.
and Colman, R. F.: Crystallization and Preliminary Diffraction Analysis of
Porcine Heart Mitochondrial NADP-Dependent Isocitrate Dehydrogenase. Acta
Crystallographica, Section D52 1024-1026 (1996).
- Soundar, S., Jennings, G. T., McAlister-Henn, L. and Colman, R. F.: Expression
of Pig Heart Mitochondrial NADP-Dependent Isocitrate Dehydrogenase in E. coli.
Protein Expression and Purification 8: 305-312 (1996).
- Colman, R. F.: Affinity Labels for NAD(P)-Specific Sites in Methods in
Enzymology 280: 186-203 (1997).
- Colman, R. F.: Chemical Arrows for Enzymatic Targets, The FASEB Journal
11: 217-226 (1997).
- Omburo, G. A., Torphy, T. J., Scott, G., Colman, R. F. and Colman, R. W.:
Inactivation of Recombinant Monocyte cAMP-Specific Phosphodiesterase by cAMP
Analog, 8-(4-Bromo-2,3-dioxobutyl)thioadenosine 3',5'-Cyclic Monophosphate.
Blood 89: 1019-1026 (1997).
- Hu, L., Borleske, B. L. and Colman, R. F.: Probing the Active Site of (alpha-Class
Rat Liver Glutathione S-Transferases Using Affinity Labelling by Monobromobimane.
Protein Science: 6: 43-52 (1997).
- Lee, T. T., Worby, C., Dixon, J. E. and Colman, R. F.: Identification of
His-141 in the Active Site of B. subtilis Adenylosuccinate Lyase by Affinity
Labelling with 6-(4-Bromo-2,3-dioxobutyl)thioadenosine 5'-Monophosphate. J.
Biol. Chem. 272: 458-465 (1997).
- Barycki, J. J. and Colman, R. F.: Identification of the Non-Substrate Steroid
Binding Site of Rat Liver Glutathione S-Transferase, Isozyme 1-1, by the Steroid
Affinity Label, 3-beta-(Iodoacetoxy)dehydroisoandrosterone. Arch. Biochem.
Biophys. 345: 16-31 (1997).
- Puri, R. N., Colman, R. F. and Colman, R. W.: Modulation of Platelet Responses
by 2-[3-Bromo-2-oxopropylthio)Adenosine 5'-Diphosphate Involves its Binding
to as well as Covalent Modification of an ADP Receptor, Aggregin. Arch. Biochem.
Biophys. 343: 140-145 (1997).
- Huang, Y.-C., Kumar, A. and Colman, R. F.: Identification of the Subunits
and Target Peptides of Pig Heart NAD-Specific Isocitrate Dehydrogenase Modified
by the Affinity Label 8-(4-Bromo-2,3-dioxobutylthio)NAD. Arch. Biochem. Biophys.
345: 207-218 (1997).
- Hu, L. and Colman, R. F.: Resonance Energy Transfer Between Sites in Rat
Liver Glutathione S-Transferase, 1-1, Selectively Modified at Cys-17 and Cys-111.
Biochemistry 36: 1635-1645 (1997).
- Chen, H., Huang, Y.-C. and Colman, R. F.: Identification of the Subunit
and Important Target Peptides of Pig Heart NAD-Dependent Isocitrate Dehydrogenase
Modified by the Affinity Label Adenosine 5'-0-[S-(4-Bromo-2,3-dioxobutyl)Thiophosphate.
Biochemistry 37: 6541-6551 (1998).
- Lee, T. T., Worby, C., Bao, Z.-Q., Dixon, J. E. and Colman, R. F.: Implication
of His-68 in the Substrate Site of Bacillus subtilis Adenylosuccinate Lyase
by Mutagenesis and Affinity Labeling with 2-[(4-Bromo-2,3-dioxobutyl)thio]adenosine
5'-Monophosphate. Biochemistry 37: 8481-8489 (1998).
- Colman, R. F.: Affinity Labeling, in The Encyclopedia of Molecular Biology,
John Wiley, New York, 76-78 (1999).
- Wang, J., Bauman, S. and Colman, R. F.: Photoaffinity Labeling of Rat Liver
Glutathione S-Transferase, 4-4, by Glutathionyl S-[4-(Succinimidyl)-Benzophenone].
Biochemistry 37: 15671-15679 (1998).
- Lee, T. T., Worby, C., Bao, Z.-Q., Dixon, J. E. and Colman, R. F.: His-68
and His-141 are Critical Contributors to the Intersubunit Catalytic Site of
Adenylosuccinate Lyase of Bacillus subtilis. Biochemistry 38: 22-32 (1999).
- Pettigrew, N. E., Moyer-Myers, M. and Colman, R. F.: Affinity Labeling
of Pig Lung Glutathione S-Transferase pi by 4-(Fluorosulfonyl)benzoic Acid.
Arch. Biochem. Biophys. 364: 107-114 (1999).
- Lee, P., Gorrell, A., Fromm, H. J. and Colman, R. F. Implication
of Arg-131 and Arg-303 in the Substrate Site of Adenylosuccinate Synthetase
of E. coli by Affinity Labeling with 6-(4-Bromo-2,3-dioxobutyl)thioadenosine
5'-Monophosphate. Biochemistry 38: 5754-5763 (1999).
- Lee, P., Gorrell, A., Fromm, H. J. and Colman, R. F. 8-(4-Bromo-2,3-dioxobutylthio)guanosine
5'-Triphosphate: A New Affinity Label for Purine Nucleotide Sites in
Proteins. Arch. Biochem. Biophys. 372: 205-213 (1999).
- Huang, Y.-C., Soundar, S. and Colman, R. F. Affinity Cleavage at
the Divalent Metal Site of Porcine NAD-Specific Isocitrate Dehydrogenase.
Protein Science 9: 104-111 (2000).
- Soundar, S., Danek, B. and Colman, R. F. Identification by Mutagenesis
of Arginines in the Substrate Binding Site of the Porcine NADP-dependent Isocitrate
Dehydrogenase. J. Biol. Chem. 275: 5606-5612 (2000).
- Wang, J., Bauman, S. and Colman, R. F. Probing Subunit Interactions
in Alpha Class Rat Liver Glutathione S-Transferase with the Photoaffinity
Label Glutathionyl S-[4-(Succinimidyl)-benzophenone]. J. Biol. Chem.
275: 5493-5503 (2000).
- Grodsky, N. B., Soundar, S. and Colman, R. F. Evaluation by Site-Directed
Mutagenesis of Aspartic Acid Residues in the Metal Site of Pig Heart NADP-dependent
Isocitrate Dehydrogenase. Biochemistry 39: 2193-2200 (2000).
- Mseeh, F., Colman, R. F. and Colman, R. W. Inactivation of Platelet
PDE2 by an Affinity Label: 8-[4-Bromo-2,3-dioxobutyl)thio]cAMP. Thrombosis
Research 98: 395-401 (2000).
- Brosius, J. L. and Colman, R. F. Key Role in Catalysis for Histidine-89
of Adenylosuccinate Lyase of Bacillus subtilis. Biochemistry 39: 13333-13343
(2000).
- Vargo, M. A. and Colman, R. F. Affinity Labeling of Rat Liver Glutathione
S-Transferase by
17 β-Iodoacetoxy-Estradiol-3-Sulfate.
J. Biol. Chem. 276: 2031-2036 (2001).
- Madhusoodanan, K. S. and Colman, R. F. Adenosine 5'-O-[S-(4-Succinimidyl-Benzophenone)
Thiophosphate]: A New Photoaffinity Label of the Allosteric Site of Glutamate
Dehydrogenase. Biochemistry 40: 1577-1586 (2001).
- Pettigrew, N.E., Brush, E.J. and Colman, R.F. 3-Methyleneoxindole: An Affinity
Label of Glutathione S-Transferase pi which Targets Tryptophan 38. Biochemistry
40: 7549-7558 (2001).
- Colman, R.F., Barycki, J.S., Vargo, M.A., Wang, J. Probing Subunit Interactions
in Glutathione S-Transferases Using Affinity Labeling. Chem-Biol. Interact.
133: 159-162 (2001).
- Pettigrew, N.E. and Colman, R.F. Heterodimers of Glutathione S-Transferase
Can Form Between Isoenzyme Classes pi and mu. Arch. Biochem. Biophys. 396:
225-230 (2001).
- Brosius, J.L. and Colman, R.F. Three Subunits Contribute Amino Acids to
the Active Site of Tetrameric Adenylosuccinate Lyase: Lys268 and
Glu 275 are Required. Biochemistry 41: 2217-2226 (2002).
- Hung, S.H., Madhusoodanan, K.S., Boyd, R.L., Baldwin, J.L., Colman, R.F.
and Colman, R.W. A Nonhydrolyzable Reactive cAMP Analog, (Sp)-8-[4-bromo-2,3-dioxobutyl)thio]
adenosine-3',5'-cyclic-S-(methyl)monophosphorothioate Irreversibly Inactivates
Human Platelet cGMP-Inhibited cAMP Phosphodiesterase at Micromolar Concentrations.
Biochemistry 41: 2962-2969 (2002).
- Lee, P. and Colman, R.F. Implication by Site-Directed Mutagenesis of Arg314
and Tyr316 in the Coenzyme Site of Pig Mitochondrial NADP-dependent
Isocitrate Dehydrogenase. Arch. Biochem. Biophys. 401: 81-90 (2002).
- Huang, Y.C. and Colman, R.F. Evaluation by Mutagenesis of the Roles of His309,
His315 and His319 in the Coenzyme Site of Pig Heart
NADP-dependent Isocitrate Dehydrogenase. Biochemistry 41: 5637-5643 (2002).
- Hung, S-H., Madhusoodanan, K.S., Beres, J.A., Boyd, R.L., Baldwin, S.L.,
Zhang, W., Colman, R.W. and Colman, R.F. A New Nonhydrolyzable Reactive cAMP
Analog, (SP)-adenosine-3',5'-cyclic-S-(4-bromo-2,3-dioxobutyl)monophosphorothioate
Irreversibly Inactivates Human Platelet cGMP-Inhibited cAMP Phosphodiesterase.
Bioorganic Chemistry 30: 16-31 (2002).
- Ceccarelli, C., Grodsky, N.B., Ariyaratne, N., Colman, R.F. and Bahnson,
B.J. Crystal Structure of Porcine Mitochondrial NADP-dependent Isocitrate
Dehydrogenase Complexed with Mn2+ and Isocitrate: Insights
into the Enzyme Mechanism. J. Biol. Chem. 277: 43454-43462 (2002).
- Palenchar, J.B. and Colman, R.F. Characterization of a Mutant Bacillus
subtilis Adenylosuccinate Lyase Equivalent to a Mutant Enzyme Found in
Human Adenylosuccinate Lyase Deficiency: Asparagine 276 Plays an Important
Structural Role. Biochemistry 42: 1831-1841 (2003).
- Palenchar, J.B., Crocco, J.M. and Colman, R.F. The Characterization of Mutant
Bacillus subtilis Adenylosuccinate Lyases Corresponding to Severe Human
Adenylosuccinate Lyase Deficiencies. Protein Science 12: 1694-1705 (2003).
- Ralat, L. A. and Colman, R. F. Monobromobimane Occupies a Distinct Xenobiotic
Site in Glutathione S-Transferase Pi. Protein Science 12 2575-2587 (2003).
- Kim, T-K., Lee, P. and Colman, R. F. Critical Role of Lys212 and Tyr140
in Porcine NADP-dependent Isocitrate Dehydrogenase. J. Biol. Chem. 278 49323-49331
(2003).
- Soundar, S., Park, J-H., Huh, T-L. and Colman, R. F. Evaluation by Mutagenesis
of the Importance of 3 Arginines in α, β and
γ Subunits of Human NAD-dependent Isocitrate Dehydrogenase.
J. Biol. Chem. 278 52146-52153 (2003).
- Huang, Y. C., Grodsky, N. B., Kim, T-K. and Colman, R. F. Ligands of the
Mn2+ Bound to Porcine Mitochondrial NADP-dependent Isocitrate Dehydrogenase.
Biochemistry 43 2821-2828 (2004).
- Vargo, M. A., Nguyen, L. and Colman, R. F. Subunit Interface Residues of
Glutathione S Transferase A1-1 Important in the Monomer/Dimer Equilibrium.
Biochemistry 43 3327-3335 (2004).
- Vargo, M. A. and Colman, R. F. Heterodimers of Wild-Type and Subunit Interface
Mutant Enzymes of Glutathione S-Transferase A1-1: Interactive or Independent
Active Sites? Protein Science 13 1586-1593 (2004).
- Segall, M. L. and Colman, R. F. Gln212, Asn270 and Arg301 Are Critical for
Catalysis by Adenylosuccinate Lyase from Bacillus subtilis. Biochemistry 43
7391-7402 (2004).
- Ralat, L. A. and Colman, R. F. GST pi Has at Least Three Distinguishable
Xenobiotic Substrate Sites Close to Its Glutathione Binding Site. J. Biol.
Chem., 279 50204-50213 (2004).
- Sivendran, S., Patterson, D., Spiegel, E., McGowan, I., Cowley, D. and Colman,
R. F. Two Novel Human Adenylosuccinate Lyases Associated with Autism and Characterization
of the Equivalent Mutant B. subtilis ASL. J. Biol. Chem., 279 53789-53797
(2004).
- Kim, T.-K. and Colman, R. F. Ser95, Asn97 and Thr78 Are Important for the
Catalytic Function of Porcine NADP-Isocitrate Dehydrogenase. Protein Science,
14 (1) 140-147 (2005).
- Lee, P. and Colman, R. F. Adenosine 2’-Monophosphate, 5’-O-[S-(4-Succinimidyl-benzophenone)thiophosphate]:
A New Photoaffinity Label for the Coenzyme Site of Porcine NADP-Specific Isocitrate
Dehydrogenase. Bioconjugate Chemistry, 16 650-659 (2005).
- Misquitta, S. A. and Colman, R. F. Communication Between the Two Active
Sites of Glutathione S-Transferase A1-1, Probed Using Wild Type-Mutant Heterodimers.
Biochemistry 44 8608-8619 (2005).
- Huang, Y. C. and Colman, R. F. Location of the Coenzyme Binding Site in
the Porcine Mitochondrial NADP-dependent Isocitrate Dehydrogenase. J. Biol.
Chem. 280 30349-30353 (2005).
- Hearne, J. and Colman, R. F. Delineation of Xenobiotic Substrate Sites in
Rat Glutathione S Transferase M1-1. Protein Science 14 2526-2536 (2005).
- Ralat, L. A., Manevich, Y., Fisher, A. B. and Colman, R. F. Direct Evidence
for the Formation of a Complex Between 1-Cysteine Peroxiredoxin and Glutathione
S-Transferase Pi with Activity Changes in Both Enzymes. Biochemistry 45 360-372
(2006).
- Hearne, J. L. and Colman, R. F. Contribution of the Mu Loop to the Structure
and Function of Rat Glutathione Transferase M1-1. Protein Science 15 1277-1289
(2006).
- O'Sullivan, S. M., McCarthy, R. M., Vargo, M. A., Colman, R. F. and Sheehan,
D. Chemical Modification at Subunit 1 of Rat Kidney Alpha Class Glutathione
Transferase with 2,3,5,6-Tetrachloro-1,4-benzoquinone: Close Structural Connectivity
Between Glutathione Conjugation Activity and Non-Substrate Ligand Binding.
Biochemical Pharmacology 71 1629-1636 (2006).
- Lee, P. and Colman, R. F. Thr373, Asp375 and Lys260 Are in the Coenzyme
Site of Porcine NADP-dependent Isocitrate Dehydrogenase. Archives of Biochem.
and Biophys. 450 183-190 (2006).
- Hearne, J. L. and Colman, R. F. Catalytically Active Monomer of Class Mu
Glutathione Transferase from Rat. Biochemistry 45 5974-5984 (2006).
- Spiegel, E. K., Colman, R. F. and Patterson, D. Adenylosuccinate Lyase Deficiency
(A Review). Molecular Genetics and Metabolism, 89 (1-2) 19-31 (2006).
- Soundar, S., O'Hagan, M., Fomulu, K. S. and Colman, R. F. Identification
of Mn2+ -Binding Aspartates from α, β and γ
Subunits of Human NAD-dependent lsocitrate Dehydrogenase.
J. Biol. Chem. 281 21073-21081 (2006).
- Colman, R.F. Insights from Affinity Labeling into Nucleotide- and Coenzyme-dependent
Enzyme Catalysis and Regulation. Letters in Drug Design and Discovery (LDDD)
3 462-480 (2006).
- Hung, S. H., Zhang, W., Pixley, R. A., Jameson, B. A., Huang, Y. C., Colman,
R. F., and Colman, R. W. New Insights from the Structure-Function Analysis
of the Catalytic Region of Human Platelet PDE3A: A Role for the Unique 44
Amino Acid Insert. J. Biol. Chem. 281
29236-29244 (2006).
- Ralat, L. A. and Colman, R. F. Identification of Tyrosine 79 in the Tocopherol
Binding Site of Glutathione S-Transferase pi. Biochemistry 45 12491-12499
(2006).
- Lee, P., and Colman, R. F. Expression, Purification and Characterization
of Stable, Recombinant Human Adenylosuccinate Lyase. Protein Expression and
Purification 51 227-234 (2007).
- Segall, M. L., Cashman, M. A. and Colman, R. F. Important Roles of Hydroxylic
Amino Acid Residues in the Function of Bacillus subtilis Adenylosuccinate
Lyase. Protein Science 16 441-448 (2007).
- Bzymek, K. P. and Colman, R. F. Role of α-Asp181, ß-Asp192 and γ-Asp190
in the Distinctive Subunits of Human NAD-Specific Isocitrate Dehydrogenase.
Biochemistry 46 5391-5397 (2007).
- Tsai, M., Koo, J., Yip, P., Colman, R. F., Segall, M. L. and Howell, L.
Substrate and Product Complexes of E.coli Adenylosuccinate Lyase Provide
New Insights into the Enzymatic Mechanism. J. Mol. Biology 370 541-554 (2007).
- Sivendran, S., Segall, M. L., Rancy, P. C. and Colman, R. F. Effect of
Asp69 and Arg310on the pK of His68, a Key Catalytic Residue of Adenylosuccinate
Lyase. Protein Science 16 1700-1707 (2007)
- Ariyananda, L. D. Z, and Colman R.F. Evaluation of Types of Interactions in Subunit Association in B. subtilis Adenylosuccinate Lyase. Biochemistry 47 2923-2934 (2008)
- Hung, S.H. , Liu, A.H., Pixley, R.A., Francis, P. Williams, L, Matsko, C.M., Barnes, K.D., Sivendran, S., Colman, R.F., and Colman, R.W. A New Non-Hydrolyzable Reactive cGMP Analogue, (Rp)-Guanosine-3’, 5’-cyclic-S-(4-Bromo-2, 3,-Dioxobutyl)monophosphorothioate, which Targets the cGMP Binding Site of Human Platelet PDE3A. Bioorganic Chemistry 36 141-147 (2008).
- Ralat, L.A., Misquitta, S. A., Manevich, Y., Fisher, A. B., and Colman, R.F. Characterization of the Complex of Glutathione S-Transferase pi and 1-Cysteine Peroxiredoxin. Arch. Biochem. Biophysics 474 109-118 (2008).
- Sivendran, S. and Colman, R.F. Effect of a New Non-Cleavable Substrate Analogue on Wild-Type and Serine Mutants in the Signature Sequence of Adenylosuccinate Lyase of Bacillus subtilis and Homo sapiens. Protein Science 17 1162-1174 (2008).
- Hartong, D.T., Dange, M, McGee, T.L., Berson, E.L., Dryja, T.P. and Colman, R.F. Insights from Retinitis Pigmentosa into the Roles of Isocitrate Dehytrogenases in the Krebs Cycle. Nature Genetics 40 (10) 1230-1234 (2008).
- Huang, Y.-c, Misquitta, S., Blond, S.Y., Adams, E. and Colman, R. F. Glutathione S-Transferase Pi: Catalytically Active Monomer and Key Residues Involved in the Electrostatic Interaction Between Subunits. J. Biological Chemistry 283 32880-32888 (2008).
- Ariyananda, L.D.Z.; Lee, P.; Antonopoulos, C. and Colman, R. F. Biochemical and Biophysical Analysis of Five Disease - Associated Human Adenylosoccinate Lyase Mutants. Biochemistry 48 (23) 5291-5302 (2009).
- Dange, M. and Colman, R. F. Each conserved Active site Tyr in the Three subunits of Human Isocitrate Dehydrogenase Has a Different Function. J. Biol. Chem 285 20520-20525 (2010).
- Thevenin, A. F., Zony, C. L., Bahnson, B. J. and Colman, R. F. Activation by Phosphorylation and Purification of Human c-Jun N-Terminal Kinase (JNK) Isoforms in milligram Amounts. Protein Expression and Purification. 75 (2) 138-46 (2011).
- Ariyananda, L. D. Z., Antonopoulos, C., Currier, J. and Colman, R. F. In Vitro Hybridization and Separation of Hybrids of Human Adenylosuccinate Lyase from Wild Type and Disease-Associated Mutant Enzymes. Biochemistry Mar. 1: 50 (8): 1336-1346 (2011).
- Thevenin, A. F., Zony, C. L., Bahnson, B. J. and Colman, R. F. GSTpi Modulates JNK Activity Through a Direct Interaction with JNK Substrate, ATF2. Protein Science 20: 834-848 (2011).