Fixation Buffers

Tyrodes-Cacodylate Buffer (mammalian Ringers) (401 mOsmols)

1 liter dd H2O
 
NaCl 6 gm
KCl 0.2 gm
Na(CH3)2AsO2.3H2O 10.7 gm
MgCl2.6H2O 0.1 gm
NaHCO3 1.0 gm
glucose 1.0 gm
CaCl2.2H2 0.2 gm
(adjust to pH 7.4 with 3.1 %HNO3)

Sodium Cacodylate contains arsenic (metabolic poison)-stabilizes solution by preventing microbial growth.

Freshwater Teleost Ringers, g/L
 
NaCl 7.4
KCL  0.36
NaHCO3 0.31
Na2HPO4 3.02
NaH2PO4 0.40
CaCl2 0.17
Amphibian Ringers, g/L
 
NaCl 6.5
KCL 0.14
NaHCO3 0.20
CaCl2 0.12